Skip to main content
. 2021 Jun 22;6(12):e142945. doi: 10.1172/jci.insight.142945

Figure 2. PH is associated with an increase in RV ACh/α7 nAChR.

Figure 2

(A) ELISA for ACh performed on homogenates from the RV at 3 wks (n = 5 Con/6 PH) and 7 wks (n = 12 Con/12 PH) and LV at 3 wks and 7 wks (n = 6 Con/6 PH). (B) AChE activity measured in homogenates from the RV and LV at both time points (n = 6 Con/6 PH). (C) Expression of α7 nAChR protein relative to Vinculin in RV at 3 wks (n = 6 Con/6 PH) and 7 wks (n = 13 Con/12 PH) normalized to controls. (D) Expression of α7 nAChR mRNA in freshly isolated RVCF and RV cardiomyocytes from control and 7 wk PH animals (n = 6 Con/6 PH). (E) Quantitated immunofluorescence intensity of α7 nAChR in nonpermeabilized isolated RVCF from 7-wk control and PH animals. (F) Summary schematic. Data are shown as mean ± SEM. Two-tailed t tests were performed between control and PH at 3 and 7 wks; Mann-Whitney U test for real-time PCR data. *P < 0.05. ACh, acetylcholine; AChE, acetylcholinesterase; CF, cardiac fibroblasts; CM, cardiomyocytes; Glyceraldehyde 3-phosphate dehydrogenase; IF, immunofluorescence; nAChR, nicotinic acetylcholine receptor; PH, pulmonary hypertension; RV, right ventricle.