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. 2021 Jun 22;6(12):e142945. doi: 10.1172/jci.insight.142945

Figure 8. RV collagen content is dependent on α7 nAChR in RV pressure overload.

Figure 8

(A) Study design of PAB model with fixed RV afterload; WT and α7 nAChR–KO mice were randomized to PAB or sham surgery and followed for 2 wks. (B and C) Gradient across the banding site (n = 14–18) assessed by echocardiography (B) and RV systolic pressure assessed by invasive hemodynamics (n = 13–18) (C). (D) RV weight normalized to respective LV weight (n = 10–18). (E) ACh levels in RV homogenates (n = 6). (F) RV end diastolic pressure. (G) Representative images of RV from WT and KO mice after sham or PA banding surgery stained with Picrosirius red. Scale bar: 20 μm. (H) RV collagen content by Sircol assay (n = 7–11). Data are shown as mean ± SEM. Two-way ANOVA followed by Bonferroni comparison test. *P < 0.05. PAB, pulmonary artery banding; RVSP, RV systolic pressure; RVEDP, RV end diastolic pressure.