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. 2021 Jun 22;6(12):e145200. doi: 10.1172/jci.insight.145200

Figure 2. Subchronic cigarette smoke exposure affects circadian focused mRNA expression analyzed by NanoString.

Figure 2

Total RNA was isolated from the lungs of mice exposed to air and cigarette smoke (CS) for 30 days. Our customized NanoString panel (circadian gene focused) was used to screen the potential targets via nCounter SPRINT Profiler. Normalization of absolute RNA count and data analysis were done by nSolver software. The overview of all the dysregulated targets is shown as a heatmap (A), and selected gene transcription changes are shown separately (B). Data are shown as mean ± SEM (n = 6; *P < 0.05, **P < 0.01, ***P < 0.001 between groups; #P < 0.05, ##P < 0.01 compared with CS-exposed WT group; &&P < 0.01 compared with WT air controls, 1-way ANOVA with Šidák correction).