Skip to main content
. 2021 Jun 22;6(12):e145200. doi: 10.1172/jci.insight.145200

Figure 3. Subchronic CS exposure affects EMT-focused mRNA expression analyzed by NanoString.

Figure 3

Total RNA was isolated from the lungs of mice exposed to air and CS for 30 days. Our customized NanoString panel (EMT gene focused) was used to screen the potential targets via nCounter SPRINT Profiler. Normalization of absolute RNA count and data analysis were performed by nSolver software. The overview of all the dysregulated targets was shown as a heatmap (A), and selected gene transcription changes were shown separately (B). Data are shown as mean ± SEM (n = 6; *P < 0.05, **P < 0.01, ***P < 0.001 between groups; #P < 0.05 compared with CS- exposed WT group; 1-way ANOVA with Šidák correction).