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. 2021 Jun 22;6(12):e145200. doi: 10.1172/jci.insight.145200

Figure 8. Acute CS exposure–induced inflammatory response was reduced by REV-ERBα agonist.

Figure 8

Proinflammatory cell influx and cytokines in BALF induced by acute CS exposure (10 days) were determined by flow cytometry and Luminex. (A) Total number of inflammatory cells was counted by Bio-Rad cell counter using Trypan blue staining. Differential inflammatory cell counts (macrophage, neutrophil, CD4/CD8 T lymphocytes) were determined (cells/mL) by flow cytometry. (B) Relative cytokines were determined by Luminex. A total of 2 technical repeats were done to calculate the final concentration. Data are shown as mean ± SEM (n = 10; *P < 0.05, **P < 0.01, ***P < 0.001, between groups; ##P < 0.01 compared with CS exposed WT group; 1-way ANOVA with Šidák correction).