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. 2021 Jun 22;6(12):e145916. doi: 10.1172/jci.insight.145916

Figure 7. Expression of ACE2 and TMPRSS2 and RBD binding in lung cells of LDR mice.

Figure 7

(A and B) Flow cytometry analysis of lung cells harvested from LDR (filled bars) and sham-treated (empty bars) mice 2 days after treatment. ACE2 (A, n = 3), TMPRSS2 (B, n = 6). Data referring to percentage of positive cells out of the indicated cell types are presented as mean ± SEM, analyzed using 2-way ANOVA followed by Bonferroni’s posttests, **P < 0.01. (C and D) Confocal microscopy scans of lung sections. (C) Sections from LDR (2 days after LDR administration) and naive mice were incubated with RBD-huFc or AChE-huFc (Fc control) and then immunostained with anti-human AF594. Staining of RBD in red and identification of nuclei by DAPI in blue. Scale bar: 50 μm. (D) Scatterplots of the fluorescence staining intensities of RBD expressed as MFI ± SEM. n = 4 mice, 5 scanned fields/lung, analyzed using unpaired t test, ***P < 0.001.