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. 2021 Apr 15;131(8):e146431. doi: 10.1172/JCI146431

Figure 9. Blocking SUMOylation suppresses EV-mediated ELNAT1–induced LN metastasis.

Figure 9

(A–C) Representative images (A) and quantification of tube formation (B) and Transwell migration (C) of HLECs treated with EVs secreted by control or ELNAT1-overexpressing UM-UC-3 cells transfected with si-NC or si-UBC9#1. Scale bar: 100 μm. A 1-way ANOVA followed by Dunnett’s test was used to assess the statistical significance. (D and E) Representative bioluminescence images (D) and quantification (E) of popliteal metastatic LNs from nude mice treated with EVs secreted by control or ELNAT1-overexpressing UM-UC-3 cells transfected with si-NC or si-UBC9#1 (n = 12). The red arrows indicate footpad tumors and metastatic LNs. A 1-way ANOVA followed by Dunnett’s test was used to assess statistical significance. (F) Quantification of popliteal LN volume (n = 12). A 1-way ANOVA followed by Dunnett’s test was used to assess statistical significance. (G and H) Representative IHC images (G) and quantification (H) of lymphatic vessels in footpad tumors (n = 12). Scale bars: 50 μm. A 1-way ANOVA followed by Dunnett’s test was used to assess statistical significance. (I) Kaplan-Meier curves show the survival of nude mice treated with EVs secreted by control or ELNAT1-overexpressing UM-UC-3 cells transfected with si-NC or si-UBC9#1. Error bars show the SD of 3 independent experiments. *P < 0.05 and **P < 0.01.