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. 2021 Apr 15;131(8):e135963. doi: 10.1172/JCI135963

Figure 2. LPCAT3 knockdown alters skeletal muscle membrane phospholipid composition in vitro.

Figure 2

(A) LPCAT3 mRNA levels in myoblasts infected with lentiviruses expressing shRNA for scrambled (SC) or LPCAT3 sequences (KD) and differentiated into myotubes (n = 4). (B–D) Lipids were extracted from C2C12 myotubes for analysis between SC and KD cells. Quantification of lyso-PC (B), PC (C), and total lyso-PC/PC (n = 6) (D). (E and F) Quantification of MC540 fluorescence in SC and KD myotubes (n = 5). Two-tailed t tests were performed. All data are represented as mean ± SEM. *P ≤ 0.05.