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. 2021 Jul 6;16(1):1010–1021. doi: 10.1515/med-2021-0299

Figure 3.

Figure 3

miR-145-5p was a target of circ_VANGL1. (a and b) RT-qPCR was used to detect the level of miR-145-5p in bladder cancer tissues (n = 36) and cells. (c) Schematic of the putative or mutant binding sites of miR-145-5p in circ_VANGL1 that predicted by starBase v2.0 tool. (d) The luciferase activity in 293T cells co-transfected with miR-145-5p mimic or NC-mimic and circ_VANGL1 wt or circ_VANGL1 mut. (e) T24 and J82 cells were transfected with si-NC, si-circ#1, or si-circ#2, respectively. And the expression level of miR-145-5p was examined by RT-qPCR. *P < 0.05.