Figure 4.

circ_VANGL1 targeted miR-145-5p to elevate cell viability, repress cell apoptosis, and reduce doxorubicin sensitivity in bladder cancer cells. (a–f) T24 and J82 cells were transfected with si-circ + NC inhibitor or si-circ + miR-145-5p inhibitor, respectively. (a) The level of miR-145-5p was determined by RT-qPCR. (b) CCK8 assay was performed to detect cell viability. (c) Cell apoptosis rate was detected by flow cytometry analysis. (d) T24 and J82 cells treated with different concentrations of doxorubicin, and cell survival rate was determined by flow cytometry analysis. (e) The IC₅₀ values for the growth of the transfected T24 and J82 cells treated with doxorubicin. (f) Western blot was used to detect the protein levels of c-caspase-3 and ki-67. *P < 0.05.