Figure 6.

Overexpression of SOX4 weakened the effect of miR-145-5p on bladder cancer cells. (a–f) T24 and J82 cells were transfected with miR-145-5p mimic + pc-NC or miR-145-5p mimic + pc-SOX2, respectively. (a) The protein level of SOX4 was determined western blot after co-transfection with miR-145-5p mimic and pc-NC or pc-SOX4. (b) CCK8 assay was performed to detect cell viability. (c) Cell apoptosis rate was detected by flow cytometry analysis. (d) T24 and J82 cells were incubated with different concentrations of doxorubicin and cell survival rate was determined by flow cytometry analysis. (e) The IC₅₀ values of cells treated with doxorubicin. (f) The protein levels of c-caspase-3 and ki-67 in the transfected cells were determined by western blot. *P < 0.05.