FIGURE 6.

JHDM1D/KDM7A is transcriptionally activated by TGF-β in breast cancer cells. (A,B) MCF7 (left panel) and T47D cells (left panel) were treated with TGF-β and harvested at indicated time points. KDM7A expression was examined by qPCR and Western blotting. (C) Human KDM7A promoter constructs were transfected into MCF7 cells followed by treatment with TGF-β. Luciferase activities were normalized by protein concentration and GFP fluorescence. Data are expressed relative luciferase activities compared with the control group. (D) Wild-type and mutant human KDM7A promoter constructs were transfected into MCF7 cells followed by treatment with TGF-β. Luciferase activities were normalized by protein concentration and GFP fluorescence. Data are expressed as relative luciferase activities compared with the control group. All experiments were performed in triplicate wells and repeated three times, and one representative experiment is shown. Data represent mean ± SD. *p < 0.05, two-tailed t-test.