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. 2021 Jun 22;12:653809. doi: 10.3389/fmicb.2021.653809

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Copyright © 2021 Zhu, Guo, Hu, Zhang, Li and Zhong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transfection of protoplasts of a virulent C. liriopes strain SJM3-2 with purified virus particles of ClPV1. (A) Colony morphology of strains SJM3-2 and a ClPV1 transfected derivative strain SJM 3-2-T5 on PDA at 27°C for 7 days. (B) Virulence test on apple fruits by inoculating with SJM 3-2 and SJM 3-2-T5. Symptoms were observed after 14 days of inoculation. (C) Average lesion lengths caused by SJM 3-2 and SJM 3-2-T5. (D) Comparison of the average radial mycelial growth rates between SJM 3-2 and SJM 3-2-T5 on PDA at 27∘C for 7 days. (E) Comparison of the conidial production of the two strains on PD at 27∘C for 5 days. (F) Virus detection in SJM 3-2 and SJM 3-2-T5 using dsRNA extraction and RT-PCR. The a, b indicate a significant difference at the P < 0.05 level of confidence.