Skip to main content
. 2020 Dec 31;79(8):847–868. doi: 10.1093/nutrit/nuaa103

Table 2.

Characteristics of included controlled intervention studies

No. Reference (year) Country Study design Population Intervention Control Duration Sleep outcome measurement Main results
Infants and toddlers (aged 0–3 y)
 1 Judge et al (2012)41 United States RCT Healthy pregnant women and their newborn babies (n = 48) Cereal bars contained fish oil (300 mg DHA/d) Corn oil Started from 24 wk gestation and continued until delivery (38–40 wk): 98–112 d Neonates’ sleep outcome including arousals, QS, AS, active-quiet sleep transition, and sleep-wake transition measured by actigraph On postnatal day 1, infants of mothers in the DHA intervention group had significantly fewer arousals in QS (t = 2.17, P < 0.05) and arousals in AS (t = 2.21, P < 0.05) compared with infants born to mothers in the placebo group. On postnatal day 2, QS: I: 12.70 ± 5.85, C: 13.70 ± 4.76; active-quiet sleep transition: I: 0.47 ± 0.30, C: 0.41 ± 0.27; sleep-wake transition: I: 51.57 ± 14.54, C: 51.70 ± 11.13. Infants of mothers in the treatment group had significantly fewer arousals in QS (F = 5.72, P < 0.05) than the placebo group when controlling for maternal total weight gain during pregnancy, because maternal weight was significantly correlated with the infant sleep measure.
 2 Boone et al (2019)46 United States RCT Children (n = 377) aged 10–16 mo born at < 35 wk gestation DHA 200 mg+AA 200 mg/d supplementation Corn oil (400 mg/d) 180 d Nocturnal and daytime sleep duration, and sleep-onset time measured by caregiver-reported BISQ Nocturnal sleep duration (h) I: 10.0 ± 1.6, C: 9.9 ± 1.5, difference in change (95%CI): 0.24 (−0.05 to 0.53), effect size = 0.16, P = 0.11; TSD (h) I: 12.1 ± 1.8, C: 12.3 ± 1.8, difference in change (95%CI): 0.14 (−0.23 to 0.51), effect size = 0.07, P = 0.32; sleep onset time (min) I: 34.4 ± 42.8, C: 32.1 ± 35.5, difference in change (95%CI): 3.50 (−4.80 to 11.79), effect size = 0.09, P = 0.35; night wakefulness (min): I: 28.6 ± 72.3, C: 26.8 ± 61.1, difference in change −0.95 (95%CI, −15.80 to 13.90), effect size = −0.01, P = 0.82. Although there is no evidence of an overall effect of DHA+AA supplementation on child sleep, exploratory post hoc analyses identified that boys and children whose caregivers had depressive symptomatology may benefit more from the supplementation.
Children (aged 4–18 y)
 3 Hysing et al (2018)50 Finland RCT Preschoolers between ages 4 and 6 y (n = 232) Three warm lunch meals per week containing fatty fish. Each meal contained 50–80 g of fatty fish. Three warm lunch meals per week containing meat. Each meal contained 50–80 g of meat. 112 d Parent-reported bedtime and rise time, time in bed, sleep latency, wake after sleep onset, sleep efficiency (ratio of duration of sleep to time in bed). Time in beda (min) I: 678 ± 42, C: 672 ± 34, change of mean score: I: −5.3 (95%CI, −11.8 to 0.3); C: −5.3 (95%CI, −11.2 to 0.6), P = 0.905. TSD (min) I: 653 ± 45, C: 644 ± 36; change of mean score, I: −1.2 (95%CI, −8.3 to 5.9), C: −1.8 (95%CI, −8.8 to 5.1), P = 0.893. Sleep latency (min): I: 23.0 ± 16.0, C: 26.3 ± 16.8; change of mean score: I: −1.9 (95%CI, −4.7 to 0.9). There were no statistically significant differences between the fish and the meat groups on any of the included sleep measures.
 4 Montgomery et al (2014)18 United Kingdom RCT Healthy children aged 7–9 y (n = 362) who were under performing in reading from mainstream UK schools Three capsules containing a total of 600 mg of algal DHA/d Three capsules /d containing corn or soybean oil, matched with the active treatment for taste and color 112 d

Behavioral and medical sleep problems were measured by CSHQ.

TSD, SL, SEff, frequency and length of wakefulness during night were measured by sleep diary and actigraphy.

Slight but nonsignificant improvements were seen in both groups for all but 1 CSHQ subscale (sleep duration). Actigraphy results showed TSD increased by 58 min more in the active group than in the control group. TSD (h): I: 3.94 ± 1.214, C: 3.88 ± 1.202, z = −0.724, P = 0.469. Sleep onset delay: I : 1.66 ± 0.676, C: 1.62 ± 0.677, z = 0.71, P = 0.478. Bedtime resistance: I : 6.99 ± 1.575, C: 7.33 ± 2.02, z = 0.998, P = 0.318. Daytime sleepiness: I: 9.56 ± 2.555, C: 9.69 ± 2.766, z = −0.128, P = 0.898. TSD: I : 40.48 ± 6.166, C: 40.87 ± 6.084, z = −0.682, P = 0.495. Actigraphy score: TSD (min): I: 639 ±  52, C: 611 ± 66, t = 0.6, P = 0.551. SEff (ratio): I: 0.8 ± 0.098, C: 0.09 ± 0.117, t = 2.000, P = 0.052. Wake episodes: I: 12.86 ± 3.93, C: 15.78 ± 6.521, t = −2.59, P = 0.013. SL (min): I: 14 ± 22, C : 25 ± 33, z = 0379, P  = 0.704.
 5 Yehuda et al, (2011)57 Israel CT Children aged 9–12 y (n = 78) and diagnosed with ADHD with onset of sleep deprivation Capsules containing 720 g/d linoleic acid and 180 g/d ALA. Placebo composed of mineral oil in identical capsule. Two capsules/d 70 d Self-reported SQ measured on a 5-point Likert-scale question SQ: I: 3.8 ± 0.7, C: 1.4 ± 0.8. Polyunsaturated acid administration was associated with significant improvement in quality of life, ability to concentrate, SQ, and hemoglobin levels.
Adults (aged > 18 y)
 6 Yehuda et al (2005)19 Israel RCT Undergraduate male students (n = 126) with test anxiety Two capsules/d containing 450 mg of ALA and linoleic acid in a 1:4 ratio Mineral oil 21 d Self-reported SQ on a 5-point Likert-scale question Participants in the intervention group reported better sleep than those who received placebo. SQ: I: 3.6 ± 1.0, C: 1.8 ± 1.1.
 7 Dretsch et al (2014)47 United States RCT US deployed soldiers aged 18–55 y (n = 106) One capsule/d containing 2500 mg EPA+DHA Identical corn oil capsules containing 12% palmitic acid, 28% oleic acid, and 56% linoleic acid 60 d Self-reported SQ was measured by PSQI. Daytime sleepiness was measured by ESS. PSQI score: I: 7.1 ± 3.4, C: 7.1 ± 3.7, effect sizes (Cohen d = 0.10), P = 0.663; ESS score I: 10.7 ± 4.5, C: 10.0 ± 4.3, Cohen d = 0.26, P = 0.247. A change in the HS-Omega-3 Index was a significant predictor of the change in ESS scores, F(1, 77) = 7.25, P = 0.009, suggesting that as omega-3 levels increased, daytime sleepiness decreased.
 8 Hansen et al (2014)48 United States RCT Male forensic patients aged 21–60 years (n = 95) from a secure forensic inpatient facility in the USA 300 gram of Atlantic salmon that contain 4.8 g of EPA+DHA was served three times a week; however during the final 4 wk of the study, only 150 g of salmon were served each time. Meat (eg, chicken, pork, beef) meals three times a week 180 d SL, SE, TSD, and actual wake time were measured by actigraph. Self-reported SQ was measured by sleep diary. Actigraph: SL (min): I: 23.30 ± 20.38, C: 30.89 ± 18.93, main effects between two groups: F = 0.198, P = 0.66; effects between pre- and post-test conditions: F = 4.14, P = 0.05; interaction between groups and conditions: F = 4.11, P = 0.05. SEff (min): I: 70.37 ± 10.33, C: 69.64 ± 7.1, effect between groups: not significant; main effects of pre- and post-conditions: F = 32.84, P < 0.001; interaction between groups and conditions: F = 1.63, P = 0.21. Actual wake time (min): I: 110.04 ± 59.09, C: 107.49 ± 31.1, with a significant effect of pre- and post-test conditions (F = 19.83, P < 0.001), and a significant increase in actual wake time from pre- to post-test (P < 0.001, d = 0.43). TSD (min): I: 328.78 ± 52.84, C: 325.53 ± 67.09; main effect of pre- and post-test conditions: F = 7.44, P = 0.008. Self-reported SQ: 3.52 ± 0.6, C: 3.41 ± 0.8, with no effect of groups (F = 0.20, P = 0.66); no effect of pre- and post-test conditions (F = 0.26, P = 0.61). Daily functioning score: I: 3.35 ± 0.86, C: 2.85 ± 0.62, with significant main effect of groups, F = 54.63, P = 0.03, no effects of the pre- and post-test conditions (F = 0.49, P = 0.49).
 9 Watanabe et al (2018)55 Japan RCT Female nurses aged 20–59 y (n = 80) and worked in inpatient wards at hospitals Omega-3 PUFA capsules containing 1200 mg EPA and 60 mg DHA per day Identical capsules contained rapeseed oil (47%), soybean oil (25%), olive oil (25%), and fish oil (3%) 91 d of capsule intake and 52 wk of follow-up Insomnia severity was measured by ISI ISI score at the week 52: I: 6.07 (95%CI, 4.96–7.18), C: 5.34 (95%CI, 4.16–6.51), group by time interaction, −0.24 (95%CI, −1.96 to 1.49), P = 0.786. Statistically significant superiority was observed in favor of the omega-3 PUFA group in terms of the ISI at 13 wk (95%CI, −6.29 to −12.56, and 0.02; P = 0.049), but no significant differences were found in other time periods.
 10 Doornbos et al (2009)51 Netherlands RCT Healthy pregnant women (n = 119) DHA 220 mg or DHA+AA 220 mg/d Soybean oil From second trimester to 3 wk after delivery Quantity and quality of sleep were assessed using sleep diaries. No between-group effects were noted. The indices of SQ did not change significantly over time in any group. In a significant regression model, F = 15.240; P < 0.001. Efficient sleep at week 4 postpartum (min) median (25th;75th percentile): I: 487 ( 420–540), C = 450 ( 370, 490), SEff (%) median (25th;75th percentile): I: 88.03 ( 81.28–90.39), C: 84.62 ( 78.22–87.50), P > 0.05 for all.
 11 Judge et al (2014)49 United States RCT (pilot trial) Pregnant women aged 18–35 years (n = 42) without self-reported significant medical history One fish oil capsule that contains 300 mg DHA, 5 d weekly Corn oil From 24 wk gestation to delivery Sleep disturbance was measured with a 5-point Likert item contained in the postpartum depressive symptomatology. Sleep disturbance at 6 mo (mean ± SD): I: 6.80 ± 3.44, C: 7.00 ±  2.67,  > 0.05.
 12 Cohen et al (2014)44 and Reed et al (2014)45 were the same study United States RCT Women aged 40–62 y (n = 355) experiencing the menopausal or postmenopausal transition Fish oil capsule containing a total omega-3 dose of 615 mg, including EPA 425 mg and DHA 100 mg, along with other assorted omega-3 PUFA (90 mg) Matching placebo capsule containing olive oil 84 d Self-reported SQ was measured by PSQI. Insomnia severity was measured by ISI. The mean PSQI score reduction was 2.1 for the omega-3 group and 1.7 for the placebo group (P = 0.09). The mean ISI reduction was 3.8 for the omega-3 group and 3.7 for the placebo group (P = 0.73).
a

Time in bed was calculated by subtracting bedtime from rise time.

Abbreviations: AA, arachidonic acid; ADHD, attention deficit hyperactivity disorder; ALA, α-linolenic acid; AS, active sleep; BISQ, Brief Infant Sleep Questionnaire; C, control group; CSHQ, Children’s Sleep Habits Questionnaire; CT, controlled trial; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; ESS, Epworth Sleepiness Scale; I, intervention group; ISI, Insomnia Severity Index; PSQI, Pittsburgh Sleep Quality Index; PUFA, polyunsaturated fatty acid; QS, quiet sleep; RCT, randomized controlled trial; SEff, sleep efficiency; SL, sleep latency; SQ, sleep quality; TSD, total sleep duration.