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. 2020 Aug 10;117(8):1908–1922. doi: 10.1093/cvr/cvaa239

Figure 3.

Figure 3

vTbx5KO mice presented with conduction defects and arrhythmia. (A) Representative ECG traces of Flox and vTbx5KO mice recorded by telemetric ECG 2 weeks upon recombination. (B) Statistical analysis of telemetric ECG measurements reveals prolonged PR and QRS intervals from 1 to 8 weeks post-rec. Line indicates Cre control mean value ± SEM 4 weeks post-rec (Cre n = 6; Flox n = 6; KO n = 7–13 biological replicates. The animal number decreased during measurements due to SCD). (C) vTbx5KO mice present with atrioventricular blocks, ventricular tachycardias, and asystoles. (D) Electrophysiological studies of isolated paced Flox, Cre, and vTbx5KO hearts show prolonged activation times from RA to RV, endocardial RV to epicardial RV, RV to septum, and RV to LV (Cre n = 10; Flox n = 8; KO n = 8 biological replicates). Data are presented as mean ± SEM. Statistical analysis was performed by (B) one-way ANOVA with Sidak’s multiple comparison post hoc test against pre-recombination data of the same group; (D) One-way ANOVA followed by Tukey’s multiple comparison post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.