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. 2021 Jul 5;48(2):164. doi: 10.3892/ijmm.2021.4996

Figure 4.

Figure 4

AS-IV inhibits NLRP3 inflammasome activation in the renal cortex of db/db mice. The mRNA levels of (A) NLRP3, (B) caspase-1, and (C) IL-1β. (D) Western blot analysis of NLRP3, pro-caspase-1 and caspase-1. The protein levels of (E) NLRP3, (F) pro-caspase-1, and (G) caspase-1 were normalized to β-actin protein level. (H) Ratio of the protein level of caspase-1 to that of pro-caspse-1. The levels of (I) IL-1β, (J) IL-6, (K) MCP-1, and (L) TNF-α in serum. Data are presented as the mean ± standard deviation. (A-C and I-L) n=6-7; (E-H) n=3. *P<0.05, **P<0.01 and ***P<0.001 vs. WT mice; #P<0.05, ##P<0.01 and ###P<0.001 vs. db/db + vehicle. WT, wild-type mice; Veh: Vehicle; AS-IV, Astragaloside IV; NLRP3, NLR family pyrin domain containing 3; IL, interleukin; TNF, tumor necrosis factor; MCP-1, monocyte chemoattractant protein-1.