Figure 2.

Immunoelectron microscopy for human OPC molecular markers. (A) NG2 label in large electron-lucent cells in the white matter. Inset shows NG2 subcellular distribution in cytoplasmic intermediate filaments and in short dilated endoplasmic reticulum cisternae in a sample of a 2-year-old male sample (PB20). (B) PDGFRα is expressed in discrete clusters in the plasma membrane of OPCs in the white matter of a 6-year-old male (PB3). (C) OPCs in the white matter labeled with A2B5. Immunogold shows the subcellular localization of this marker in the cytoplasmic portion of the cells and is also associated with the endoplasmic reticulum in the white matter of a 5-year-old female (PB19). N, Nucleus; ER, endoplasmic reticulum; mA, myelinated axon; m, mitochondria. Scale bars: panoramic micrographs, 1 μm; insets: 250 nm.