Table 2.
Antibody and concentrations used.
| Antigen | Host species | Antibody | IF concentration | IG concentration | Cell type labeling |
|---|---|---|---|---|---|
| NG2 | Rabbit | ab5320 (Abcam) | 1:200 | 1:100 | OPCS |
| PDGFRα | Mouse | 556001 (BD Biosciences) | 1:50 | 1:50 | OPCs |
| A2B5 | Mouse | MAB312 (Millipore) | 1:100 | 1:50 | OPCS |
| Olig2 | Rabbit | AB9610 (Millipore) | 1:300 | 1:150 | OPCs, pre-OLs, m-OLs |
| BCAS1 | Mouse | sc-136342 (Santa Cruz) | 1:200 | 1:150 | pre-OLs |
| Sox10 | Rabbit | ab155279 (Abcam) | 1:300 | 1:150 | pre-OLs |
| APC (CC1) | Mouse | ab16794 Abcam | 1:100 | 1:50 | m-OLs |
| MAG | Mouse | Mab15667 (Millipore) | 1:100 | 1:100 | myelin |
| MBP | Rat | ab7349 (Abcam) | 1:100 | 1:100 | myelin |
| GFAP | Mouse | Millipore | 1:200 | 1:100 | Astrocytes |