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. 2021 Jun 4;49(W1):W72–W79. doi: 10.1093/nar/gkab393

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Schematic representation of the fragmentation procedure applied to circular RNA sequences. The sequence fragmentation approach applied to any linear RNA is based on the division of RNA sequences with length into overlapping fragments with length . These fragments expand from the 5' to the 3' end of sequence with a step equal to the half of the fragment length (). Similarly, in order to avoid uncovered parts in the 3'end, the fragmentation procedure is also applied in reverse, expanding from the 3' to the 5' end of the sequence. The same process is applied to circRNAs, but with three additional fragments that cover the back-splicing junction (BS), one that is centered exactly on the BS and two more fragments starting at positions and , respectively.

Inline graphic — Schematic representation of the fragmentation procedure applied to circular RNA sequences. The sequence fragmentation approach applied to any linear RNA is based on the division of RNA sequences with length into overlapping fragments with length . These fragments expand from the 5' to the 3' end of sequence with a step equal to the half of the fragment length (). Similarly, in order to avoid uncovered parts in the 3'end, the fragmentation procedure is also applied in reverse, expanding from the 3' to the 5' end of the sequence. The same process is applied to circRNAs, but with three additional fragments that cover the back-splicing junction (BS), one that is centered exactly on the BS and two more fragments starting at positions and , respectively.