Figure 4.

Moderate force contractions fail to mitigate glucocorticoid receptor (GR) translocation. Unilateral, moderate force muscle contractions were induced in mice before saline or dexamethasone administration. Analysis was conducted on the tibialis anterior (TA) muscle. GR content in the cytosolic-enriched fraction (A), nuclear-enriched fraction (B), and the nuclear to cytosolic ratio (C) was determined by Western blot analysis. D: purity of cytosolic and nuclear fractions. E: representative Western blots. F and G: REDD1 protein content was determined by Western blot analysis. Results are means ± SD. n = 7 male mice per group generated from three experimental replicates. Analyzed by two-way ANOVA with Sidak’s post hoc. Significance at P ≤ 0.05. CONT., contraction; DEX, dexamethasone; ME, main effect; REDD1, regulated in development and DNA damage 1.