FIG 1.

Both Cgmip1Δ and Scmip1Δ show typical petite phenotypes. (A) Small colonies and loss of mitochondrial reductive power, as indicated by lack of tetrazolium dye reduction; (B) lack of growth in alternative carbon sources such as glycerol and absence of mitochondrial DNA (mtDNA) as determined by optical density and qPCR (n = 3 for each type of experiment, color by mean); (C) high resistance to azoles, including fluconazole (FL), voriconazole (VC), and clotrimazole (CL), and (D) overexpression of efflux pump-related genes (mean ± SD, n = 3 independent experiments with 3 technical replicates each). C.g., C. glabrata; S.c., S. cerevisiae.