FIG 1.
Growth phenotype of mutants inactivated for one or more elongation PG synthase. (A) Schematic highlighting the two different modes of cell elongation used by rod-shaped cells. Green color depicts areas of new cell wall insertion. (B) Overnight cultures of MB001 (WT) and its indicated derivatives HL18 (ΔponA), JS20 (ΔponB), HL31 (ΔrodA), JS36 (ΔponA ΔponB), JS38 (ΔrodA ΔponA), and JS21(attB1::pJWS117) (ΔrodA ΔponB [Psod::riboE1-mScar-ponB]) grown in BHIS medium at 30°C were normalized to an OD600 of 0.5 and serially diluted. An aliquot of each dilution (5 μl) was then spotted onto BHI or BHIS agar medium. Plates were incubated at 30°C for 24 h and photographed. The expression construct pJWS117 encodes an mScar-PBP1b fusion with the theophylline-inducible riboE1 riboswitch (50) controlling its translation. Inducer was not added to the medium in order to deplete JS21(attB1::pJWS117) cells of PBP1b.