TABLE 1.
Expression of genes encoding PG synthases in mutant backgrounds
Gene | Normalized gene expression relative to WT in strains with mutation of:a |
||||
---|---|---|---|---|---|
ΔponA | ΔponB | ΔrodA | ΔponA ΔponB | ΔponA ΔrodA | |
ponA | 0.001 | 1.010 | 1.096 | 0.001 | 0.001 |
ponB | 1.023 | 0.001 | 1.119 | 0.001 | 1.145 |
rodA | 0.993 | 1.022 | 0.001 | 1.135 | 0.002 |
ftsW | 1.102 | 1.047 | 1.040 | 1.143 | 1.183 |
pbpA/pbp2b | 1.042 | 1.028 | 1.367 | 1.145 | 1.575 |
ftsI | 1.078 | 1.007 | 0.985 | 1.287 | 1.247 |
pbp/pbp2a | 1.188 | 1.277 | 1.241 | 1.287 | 1.549 |
Fold change of normalized read count for each of the listed genes in each deletion mutant relative to the read count from wild-type (MB001) cells. Raw read counts for each gene were normalized across samples to account for sample coverage. Tabulated data represent the average of duplicate RNA-seq runs for each strain.