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Pgp3, GlgA, CT143, and CT144 colocalize to the same globular structure. WT L2-infected McCoy cells were fixed with PFA at 24 hpi, stained with different combination of anti-Pgp3, GlgA, CT143, CT144, and IncA, and analyzed by confocal microscopy. Anti-IncA was used to identify the boundary of the chlamydial inclusion membrane. Bar, 5 μm. Note that each of the plasmid-regulated proteins is contained within the same globular structure. Bar, 5 μm.
