FIG 5.

The formation of globular structures and GlgA and Pgp3 secretion to the host cytosol are dependent on Pgp4. (A) The Pgp4 ORF was replaced by the Pgp3 or GlgA ORF. (B) The Pgp4 promoter drove the expression of Pgp3. In the absence of Pgp4, Pgp3 was highly expressed but there was no cytosolic secretion observed. (C) The expression of GlgA was driven by the Pgp4 promoter. In the absence of Pgp4, GlgA was highly expressed but expression was also restricted to chlamydial organisms. Bar, 10 μm.