Skip to main content
. 2021 Jun 1;12(3):e00262-21. doi: 10.1128/mBio.00262-21

FIG 4.

FIG 4

PA2066 and PA0256 are toxic when expressed in E. coli cytoplasm. BL21 cytoplasmic toxicity assay: survival after overnight growth on 100 mM IPTG inducing agar. When grown on 2% glucose inhibiting agar in parallel, all samples grew the same. (A) Toxicity assay of PA2066, n = 5; the mean with standard error of the mean (SEM) for pET22b is 4,200,000 ± 503,433, for pET22b:HA-PA2066 is 31,133 ± 11,364, and for pET22b:PA2066-HA is 4,380,000 ± 827,419. (B) Toxicity assay of PA0256, n = 4; the mean with SEM for pET22b is 3,775,000 ± 670,044, for pET22b:HA-PA0256 is 27,908 ± 15,459, and for pET22b:PA0256-HA is 34,608 ± 10,347. (C) Toxicity assay of PA3440, n = 3; the mean with SEM for pET22b is 9,000,001 ± 1,071,518, for pET22b:HA-PA3440 is 5,455,556 ± 577,457, and for pET22b:PA3440-HA is 6,744,444 ± 1,313,228. Statistical testing was conducted by one-way analysis of variance (ANOVA) with Dunnett multiple-comparison test and family-wise significance and confidence level set to P = 0.05; each effector compared to pET22b control. ***, P < 0.001; ns, not significant. n is number of replicate toxicity assays.