TABLE 2.
Y. pseudotuberculosis primers used in this study
| Primer | Primer sequencea | Reference |
|---|---|---|
| FiscR_cds | gatatcgaattcctgcagcccggggGCTCCTTAAATTTAGCCATGGC | This work |
| RiscR_cds | ggtctttgtagtcTGCGCGCAGATTGACGTTAATC | This work |
| F3xFLAG | cgtcaatctgcgcgcaGACTACAAAGACCATGAC | This work |
| R3xFLAG | ccgcaaattctgcttaCTCGAGTCCACCTTTATC | This work |
| F3’iscR | aggtggactcgagTAAGCAGAATTTGCGGAATTTTAC | This work |
| R3’iscR | ggtggcggccgctctagaactagtgCCTTCAACATAGTTGAAGC | This work |
| F5’ΔsufABCDSE | cgaattcctgcagcccggggTCAATCCTCGTTTTGCGC | This work |
| R5’ΔsufABCDSE | cctccagaccAGAAAATGTCCCAACTGATTC | This work |
| F5’ΔDN756_21815- DN756_21820 |
cgaattcctgcagcccggggGTTGAGTTCTGAAAGAACAATTGTG | This work |
| R5’ΔDN756_21815_21820 | ggcgaccttcTTTGCGGTTGGCTTCGATG | This work |
| F3’ΔsufABCDSE | gacattttctGGTCTGGAGGCGATGATC | This work |
| R3’ΔsufABCDSE | agggaacaaaagctggagctATAGTCAGTACTGACCCCG | This work |
| F3’ΔDN756_21815-21820 | caaccgcaaaGAAGGTCGCCTCGATAAAG | This work |
| R3’ΔDN756_21815_21820 | agggaacaaaagctggagctTTAACTGATCTGCCAGAATTAC | This work |
| qPCR_lcrF_F | GGAGTGATTTTCCGTCAGTA | 8 |
| qPCR_lcrF_R | CTCCATAAATTTTTGCAACC | 8 |
| qPCR_iscR_F | CAGGGCGGAAATCGCTGCCT | 9 |
| qPCR_iscR_R | ATTAGCCGTTGCGGCGCCTAT | 9 |
| qPCR_sufA_F | CGCAAATTACGCGGCTTATGC | This work |
| qPCR_sufA_R | GGCAGGCTCTTTAGCCATATC | This work |
| qPCR_iscS_F | CGACGCCAGTAGATCCGCGT | 19 |
| qPCR_iscS_R | ACGAGGGTCTGCACCCACCA | 19 |
| qPCR_yopE_F | CCATAAACCGGTGGTGAC | 98 |
| qPCR_yopE_R | CTTGGCATTGAGTGATACTG | 98 |
| qPCR_16s_F | AGCCAGCGGACCACATAAAG | 25 |
| qPCR_16s_R | AGTTGCAGACTCCAATCCGG | 25 |
| EMSA_suf_F | tttttCTCGAGGAGTGTTTTTTCTTTTAGACC | This work |
| EMSA_suf_R | tttttGGATCCTAAACGTTTTCAGGTTGAA | This work |
| EMSA_dusBfis_F | tttttCTCGAGGTATCGATAATATTTCAGTATTAAC | This work |
| EMSA_dusBfis_R | tttttGGATCCGTAGAAATATATCTGTCACACATC | This work |
| EMSA_ail_F | tttttCTCGAGCTGTCACCGTCCTGG | This work |
| EMSA_ail_R | tttttGGATCCGACTAAAGTGGCCAGCC | This work |
| EMSA_sodB_F | tttttCTCGAGTAACTGACGGTCCG | This work |
| EMSA_sodB_R | tttttGGATCCGGTGTGGGGTGAA | This work |
| EMSA_katA1_F | ccgCTCGAGTATTGCTCTCTCATTGTTCGTTATG | This work |
| EMSA_katA1_R | cgcGGATCCATTTGAAATTATGATCATTTGAAATACTGTGC | This work |
| EMSA_katA2_F | ccgCTCGAGGATCGTCAGTTCCCACAG | This work |
| EMSA_katA2_R | cgcGGATCCCCCCACTAGTTGTAGTCG | This work |
| NdeI-iscR_F | GGAATTCCATATGAGACTGACATCTAAAGGGCGC | This work |
| BamHI-His6-IscR_R | GCGGGATCCTTAGTGGTGGTGGTGGTGGTGAGCGCGTAACTTAACGTCGATCGC | This work |
a
Uppercase nucleotides specify primer that anneals to target for molecular cloning; lowercase nucleotides depict complementary sequence for NEB Gibson Assembly or extra nucleotides to facilitate efficient restriction digestion. The XhoI and BamHI restriction sites are underlined.