FIG 7.

Loss of PGRMC1/2 by siRNA knockdown during progesterone treatment leads to a significantly decreased level of HEV replication. (A) Experimental regimen. (B) Percentage fold change in HEV negative-strand RNA levels; the Friedman test of differences among the multiple groups rendered a chi-square value of 8.40, which was significant (P = 0.03). (C) Infectious HEV titer (FFU/ml). The data represent an average ± SEM of panels B (n = 4) and C (n = 3 independent experiments); Student’s t test. N.S, nonsignificant; siCnt, scramble control siRNA transfected cells; siPGRMC1 + 2, PGRMC1/2 siRNA transfected cells; 80 nM PRO = 25 ng/ml progesterone final concentration.