TABLE 2.
Oligonucleotide primers used in this study
| IDa | Sequence (5′–3′) | Purpose |
|---|---|---|
| IFN-α FP | GCCATCTCTGTCCTCCATGAG | qPCR |
| IFN-α RP | TCACACAGGCTTCCAAGTCATT | qPCR |
| IFN-β FP | AGTAGGCGACACTGTTCGTG | qPCR |
| IFN-β RP | GCCTCCCATTCAATTGCCAC | qPCR |
| IFN-λ1 FP | AAAAAGGAGTCCGCTGGCTG | qPCR |
| IFN-λ1 RP | TCAGACACAGGTTCCCATCG | qPCR |
| TNF-α FP | GCTGCACTTTGGAGTGATCG | qPCR |
| TNF-α RP | GAGGGTTTGCTACAACATGGG | qPCR |
| IL-6 FP | CAATGAGGAGACTTGCCTGG | qPCR |
| IL-6 RP | TGGGTCAGGGGTGGTTATTG | qPCR |
| IL-8 FP | GGCTGGAGAGCTACACAAGA | qPCR |
| IL-8 RP | ACCCATCTCTCCTTGGGGTC | qPCR |
| IL-1β FP | AACCTCTTCGAGGCACAAGG | qPCR |
| IL-1β RP | GGCGAGCTCAGGTACTTCTG | qPCR |
| IL-22 FP | GCCCTATATCACCAACCGCA | qPCR |
| IL-22 RP | CGCTCACTCATACTGACTCCG | qPCR |
| RPS18 FP | TGATCCCTGAAAAGTTCCAGCA | qPCR |
| RPS18 RP | CTTCGGCCCACACCCTTAAT | qPCR |
| PR-B FP | ATGACTGAGCTGAAGGCAAAGG | CDS PCR |
| PR-B RP | CTTTTTATGAAAGAGAAGGGGTTTCACC | CDS PCR |
| GPER FP | ATGGATGTGACTTCCCAAGCCC | CDS PCR |
| GPER RP | CACGGCACTGCTGAACCTCACAT | CDS PCR |
| Tag+HEV-FP | CGGTCATGGTGGCGAATAAGGTGGTTTCTGGGGTGAC | HEV-ve strand cDNA synthesis |
| Tag | CGGTCATGGTGGCGAATAA | HEV-ve strand qPCR FP |
| HEV-FP | GGTGGTTTCTGGGGTGAC | HEV qPCR |
| HEV-RP | AGGGGTTGGTTGGATGAA | HEV qPCR |
| HEV-Probe | 5′FAM/TGATTCTCAGCCCTTCGC/3′BHQ | HEV qPCR |
a
FP, forward primer; RP, reverse primer.