Association of rDNA and YFP-ComEA without DNase treatment. In each group of four images (a to d in panel A and a to h in panel B), the top and bottom pairs show views of a single cell, related by 180° rotations. The left-hand images in each group show the superimposed YFP, rhodamine, and CFP channels, where CFP delineates the cytoplasmic volume. The right-hand images show only the YFP and rhodamine channels. Panel A shows images collected after 1-min incubation of the cells with rDNA and panel B shows images collected after 10 min.