FIG 3.

TPP⁺ accumulation in K. pneumoniae MGH78578 and its isogenic mutant MGH78578 ΔsoxS. All measurements were performed in 100 mM NaPi buffer containing 0.1% glucose, pH 8.0. Concentrated cell suspensions were added to obtain OD₆₀₀ of 1. Final concentrations of NMP (μg/ml) are indicated in panels B and C. The final concentrations of polymixin B (PMB) are indicated in the figure: 3, 6 and 9 μg/ml (A), 50 μg/ml (B), or 6 and 4 μg/ml for wt and ΔsoxS cells, respectively (C).