TABLE 1.
The PKS23 biosynthetic gene cluster in Cladonia rangiferina
| ORFa | Size (aa) | BLASTP homologb | Identity (%) | Conserved domain | E value |
|---|---|---|---|---|---|
| 06811 | 744 | MFS monosaccharide transporter | 71 | Sugar transporter (Pfam00083) | 1e−105 |
| 06812 | 306 | Export control protein CHS7-like | 68 | Chitin synthase III catalytic subunit (Pfam12271) | 1e−131 |
| 06813 | 495 | TFIIH complex p47 subunit | 58 | TFIIH complex subunit Ssl1-like (Pfam04056) | 1e−82 |
| 06814 | 549 | MFS multidrug transporter (atr4) | 50 | Major facilitator superfamily (Pfam07690) | 9e−31 |
| 06815 | 346 | Trt5, O-methyltransferase (atr3) | 37 | SAM-dependent methyltransferase (Pfam08241) | 1e−04 |
| 06816 | 508 | Cytochrome P450 monooxygenase (atr2) | 43 | Cytochrome P450 (Pfam00067) | 3e−57 |
| 06817 | 2,513 | Nonreducing polyketide synthase (atr1) | 44 | SAT-KS-AT-PT-ACP-MT-TEc | |
| 06818 | 472 | Hypothetical protein | 33 | Phosphotransferase enzyme family (Pfam01636) | 4e−08 |
| 06819 | 2,377 | Reducing polyketide synthase (R-PKS) | 40 | KS-AT-DH-ER-KR-ACPc | |
| 06820 | 184 | Hypothetical protein | 40 | Domain of unknown function, DUF3237 (Pfam11578) | 5e−22 |
| 06821 | 301 | Hypothetical protein | 34 | Domain of unknown function, DUF2306 (Pfam10067) | 2e−09 |
| 06822 | 236 | Hypothetical protein | 28 | Not detected | |
| 06823 | 356 | Hypothetical protein | 32 | Not detected | |
| 06824 | 565 | Putative dimethylaniline monooxygenase | 41 | Flavin-binding monooxygenase-like (Pfam00743) | 3e−14 |
a
ORF, C. rangiferina open reading frame. The syntenic core genes are highlighted in boldface.
b
For BLAST searches, the NCBI nonredundant protein sequence database for four Aspergillus species was used: Aspergillus fumigatus (taxid 746128), A. nidulans (taxid 162425), A. niger (taxid 5061), and A. terreus (taxid 33178).
c
For PKS domain architecture, see Data Set S2 in the supplemental material.