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. 2021 May 11;12(3):e00769-21. doi: 10.1128/mBio.00769-21

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Copyright © 2021 Liu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Exos are differentially induced and detected in mouse plasma and EC culture media in response to rickettsial infection. (a) Expression of indicated protein markers (i.e., 30, 60, and 90 μg of plsExos proteins) was examined using Western immunoblotting. (b and c) The concentration of plsExos and ECExos was analyzed using NTA (n = 5 per group). (d and e) The concentration of exosomal total protein was determined using the micro-bicinchoninic acid (microBCA) protein assay (n = 5 per group). Statistical significance was determined using Student’s t test. *, P < 0.05.