TABLE 2.
Intracellular nucleotide concentrationsa
| ATP (μM) | ADP (μM) | AMP (μM) | GTP (μM) | GDP (μM) | GMP (μM) | Cyclic di-GMP (μM) | ppGpp (μM) | ||
|---|---|---|---|---|---|---|---|---|---|
| WT pheS/pheT | relA+-A | 338 | 428 | 991 | 51 | 237 | 1,100 | 1.1 | 10 |
| relA+-B | 266 | 315 | 665 | 43 | 238 | 908 | 1.8 | 6.1 | |
| ΔrelA-A | 238 | 219 | 245 | 32 | 117 | 265 | 0.5 | 2.8 | |
| ΔrelA-B | 185 | 155 | 218 | 24 | 88 | 247 | 1.0 | 2.6 | |
| pheT G318W | relA+-A | 252 | 329 | 745 | 36 | 187 | 925 | 1.4 | 3.1 |
| relA+-B | 274 | 344 | 797 | 40 | 238 | 908 | 1.2 | 7.1 | |
| ΔrelA-A | 243 | 225 | 175 | 31 | 126 | 201 | 0.7 | 2.2 | |
| ΔrelA-B | 245 | 302 | 421 | 32 | 172 | 513 | 0.8 | 3.8 | |
| pheS A294G | relA+-A | 195 | 174 | 201 | 32 | 160 | 309 | 3.7 | 3.2 |
| relA+-B | 161 | 162 | 253 | 22 | 143 | 419 | 1.9 | 2.0 | |
| ΔrelA-A | 206 | 187 | 229 | 26 | 109 | 244 | 0.6 | 2.3 | |
| ΔrelA-B | 133 | 136 | 179 | 16 | 85 | 177 | 0.7 | 1.9 | |
| pheS A294S | relA+-A | 179 | 181 | 247 | 31 | 165 | 462 | 3.0 | 3.9 |
| relA+-B | 152 | 188 | 279 | 26 | 158 | 455 | 7.0 | 3.5 | |
| ΔrelA-A | 194 | 176 | 234 | 25 | 116 | 289 | 0.4 | 1.9 | |
| ΔrelA-B | 101 | 104 | 141 | 13 | 89 | 159 | 1.2 | 2.7 |
a
Both E. coli MG1655 relA+ and ΔrelA strains were grown in medium A (containing all 20 amino acids) and medium B (containing no Phe, a lower Tyr concentration, and added m-Tyr) for all 4 PheRS strains. Concentrations are an average from biological triplicates and experimental triplicates.