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Actin cage structures are formed in cells infected with amastigotes. Infected Vero cells during the intracellular amastigote stage were fixed and incubated with phalloidin-TRITC for actin cytoskeleton observations. (A) Noninfected cell. (B and C) Cells containing amastigotes (arrowheads). Actin cages are clearly visible (Arrows). Images were obtained by confocal microscopy and reconstructed using Imaris software. Size bar, 15 μm.
