FIG 4.

Latency reactivation testing using the VIP-SPOT assay. (A) Detection of reactivation of HIV-1 in J-Lat cells. Comparison of various latency reversing agents and the different readouts that can be used to detect the activity of the LRAs in this latency model, i.e., percentage of green fluorescent protein-positive (GFP⁺) cells and viability analyzed by flow cytometry, cell-associated HIV-1 RNA measured by ddPCR, and frequency of HIV antigen-producing cells detected by VIP-SPOT assay. (B) Comparison of the magnitude of the reactivation effect by different latency reversing agents (LRAs) according to HIV antigen production in primary CD4⁺ T cells measured by VIP-SPOT. Representative experiment included 2 samples from HIV-infected individuals and 1 from a noninfected (HIV⁻) donor.