FIG 3.

Sequence properties of Botrytis cinerea narna-like viruses. (A) Amino acid sequence alignment of narnavirus RdRp showing conserved motifs I to VIII of Botrytis cinerea narnavirus 4 (BcNV4) and Saccharomyces 20S RNA narnavirus (SCV20; AF039063). The conserved motifs of the RdRps are shaded with light and dark gray colors. Asterisks indicate identical amino acid residues, and colons indicate similar residues. Amino acids in parentheses show the positions of amino acid residues that are not listed. (B) Schematic representation of Botrytis cinerea binarnavirus 1 (BcBNV1), -3, -4, and -5 RNA genomes showing location of ORFs. (C) Percent identity matrix generated by Clustal Omega 2.1. Identities from higher to lower are labeled from dark green to dark red, respectively. (D) Schematic representation of BcBNV2 RNA genome showing the locations of ORFs. (E) Alignment of the conserved sequences of the 5′ and 3′ ends of BcBNV2 segments 1 and 2. (F) Alignment of the hypothetical protein sequences showing conserved stretches included a GDD triplet (EVGDDR).