FIG 8.

Sequence properties of Botrytis cinerea bocivirus 1. (A) Schematic representation of Botrytis cinerea bocivirus 1 (BcBV1) vcRNA (viral RNA complementary strand) segments showing the locations of ORFs, alignment of the conserved sequences of the 5′ and 3′ ends of BcBV1 RNA1, -2, and -3, and the panhandle structures formed by the 5′ and 3′ termini of each RNA segment. (B) Alignment of the conserved region of movement protein (MP)-like proteins of Grapevine-associated cogu-like virus 2 (GsCLV2; MN520754) and GsCLV3 (MN520757), Laurel Lake virus (LLV; KX774630), and BcBV1 RNA2. (C) Phylogenetic tree of MP-associated proteins computed by IQ-TREE stochastic algorithm to infer phylogenetic trees by maximum likelihood (model of substitution: VT+G4). A consensus tree was constructed from 1,000 bootstrap trees (log likelihood of consensus tree, −9944.852649). All bootstrap values (%) of >65 are represented at each node of the tree. Branch lengths are proportional to the number of amino acid substitutions and are measured by a scale bar. (D) Phylogenetic tree of nucleocapsid proteins (NCP) computed by using an IQ-TREE stochastic algorithm to infer phylogenetic trees by maximum likelihood (model of substitution: LG+G4). A consensus tree was constructed from 1,000 bootstrap trees (log likelihood of consensus tree, −6678.055394).