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Gallein avoids off-target toxicity. (A) HEK cell cytotoxicity assay. Viability determined via trypan blue staining following 48 h of incubation with compounds. (B) Counterscreen for inhibition of human protein kinases by 10 μM gallein via radioactive ([33P]ATP) filter-binding assay performed by the University of Dundee MRC PPU (26). (A) ns, P > 0.05; *, P < 0.05 (one-way ANOVA, Tukey’s multiple-comparison test, n = 3).
