Differences in fungal diversity and total fungal load among stool samples collected at 3 months and 1 year of age from infants in the CHILD Cohort Study determined using ITS-2 amplicon sequencing and qPCR, respectively. (A) Beta diversity within subjects over time is no more similar than beta diversity between samples at the same time point based on Bray-Curtis dissimilarity. (B) Total fungal load in stool at 3 months and 1 year of age shown as ITS-2 copy number on a log scale. (C) Fungal alpha diversity (top, Shannon; bottom, Chao1) at 3 months and 1 year of age. (D) Relative abundances of the top 20 most abundant fungal ASVs annotated to genus level in stool samples collected at 3 months and 1 year of age from infants in the CHILD Cohort Study (ASVs not annotated to the level shown were removed). (E) Heat map of top 20 differentially abundant (by P value) ASVs found between 3-month and 1-year stool samples. Genus colors correspond to labels in the key in panel D. (F) Principal-coordinate plot of all samples based on Bray-Curtis dissimilarity of variance stabilized ASV count data and colored according to the relative abundance of Saccharomyces cerevisiae in the sample. Shapes indicate age at sample collection (R2 = 0.0253; P = 0.001). Dots and lines represent the sample mean and range, respectively, in all Tufte plots.