FIG 2.

Casein kinase 1 is involved in temperature compensation of the Neurospora clock. (A, left) Representative photos of race tubes used to evaluate effects of 6-DMAP treatment on conidiation rhythms in the wild-type strain over a range of temperatures. (Right) Plot of period versus temperature at indicated 6-DMAP concentrations. Error bars are standard errors of means (n = 3). (B, left) Representative photos of race tubes used to evaluate effects of PF670462 treatment on conidiation rhythms of the wild-type strain. (Right) Plot of period versus temperature at indicated PF670462 concentrations. Error bars are standard errors of means (n = 3). (C) Diagram depicting the strategy for creation of the homokaryotic ck1a^H123Y strain by homologous recombination (see Materials and Methods for details). (D) Western blot of FRQ and CK1a protein levels in the wild-type and ck1a^H123Y strains. (E, left) Representative photos of race tubes used to evaluate conidiation rhythms of the WT (ck1a^WT) and ck1a^H123Y mutant. (Right) Plot of period versus temperature for wild-type and mutant strains. Error bars indicate standard errors of means (n = 3).