FIG 2.
BB3749 encodes the HtrA family serine protease DegP, and its catalytic activity is required for efficient FhaB processing. (A) Diagram of DegPBb with the catalytic triad (HDS) and two PDZ domains indicated. The catalytic serine at position 237 was changed to an alanine to generate the inactive (iDegP) strain. (B) Western blot analysis of WCL from cultures grown for 4 h in BvgAS-activating medium (SS) after overnight growth in BvgAS-inactivating medium (SS + 20mM MgSO4). (C) Ratio of processed FHA′ to full-length FhaB in the Western blot in panel B and three similar blots. The significant difference of P < 0.05 (as determined by unpaired two-tailed t test) is indicated by an asterisk.