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. 2021 Jul 6;10:e69708. doi: 10.7554/eLife.69708

Figure 1. Suitability of TIME cells as a model to study hantavirus entry and the generation of knockout cells.

(a) Upper panels, total flow cytometry plots of HUVEC and TIME cells stained for endothelial cell markers PECAM and von Willebrand factor (vWF). Medium and lower panels, surface flow cytometry plots of HUVEC and TIME cells stained for PCDH1, β3 integrin, DAF, β1 integrin. (b) Surface flow cytometry of wild-type (WT) and knockout (KO) TIME cells stained as above. Histograms of WT cells are shown in gray; single- and double-KO cells are shown in color. (c) Western blot analysis of WT TIME cells and KO cells ± cDNA. β-Actin was used as a loading control.

Figure 1—source data 1. Original blot of WT TIME cells and KO cells ± cDNA.

Figure 1.

Figure 1—figure supplement 1. Sanger sequences retrieved from the targeted genomic loci for each knockout cell population.

Figure 1—figure supplement 1.

Sequences of interests were amplified by PCR and then TA-cloned into the pGEM-T vector. For each knockout cell population, 15–20 clones were subjected to Sanger sequencing. All sequenced clones showed an indel at the targeted site, resulting in a frameshift that brought one or more stop codons into frame.