Figure 5. K284 reduces AD-like skin inflammation in AD-like RHS models. RHS model inserts were cultured for AD cocktail for 6 days. (A) H&E staining was performed to confirm the histological changes of AD-RHS. The bar graph indicates the thickness of the epidermal thickness (n=3). (B and C) The levels of IL-1β, IL-6, CHI3L1 and LTF in AD-RHS tissues were measured by qPCR and ELISA (n=3). (D) Expressions of COX-2, CHI3L1, p-p65, p65, p-IκBα and IκBα were observed by Western blot analysis in AD-RHS tissues.

^#Control vs. AD cocktail and ^*AD cocktail vs. AD cocktail+K284. ^#,*p<0.05, ^##,**p<0.01 and ^###,***p<0.001.