Figure 3. Homeostatic proliferation of porcine T cells induced by either human or mouse IL-7 in vitro. The CFSE-labeled porcine PBMCs were seeded in 48-well plates and treated with either human or mouse IL-7. These cells were cultured and harvested on days 3, 7, and 14. The CFSE profiles were gated on CD4⁺ or CD8⁺ T cell staining. Histograms of CFSE-labeled CD4⁺ or CD8⁺ or CD4⁺CD8⁺T cells are shown. We obtained similar results from other pigs of the same strain. Each experiment involved 3 independent experiments.