Figure 4. Influence of human IL-7 on the survival of porcine T cells. Porcine PBMCs and mouse splenocytes were cultured in 48-well plates for 5 days. The frequencies and numbers of PI⁺ cells were measured by flow cytometry. (A) The fold change of percentage of PI⁺ lymphocytes are plotted at the indicated concentration of human IL-7. The percentage of PI⁺ porcine CD4⁺ (B) or CD8⁺ (C) or CD4⁺CD8⁺ (D) T cells is shown. Mouse splenocytes were used as a positive control in this experiment. These experiments were done in quadruplicates, in 3 independent experiments. Error bars represent SD. The statistical significance was measured by t-test.

^*p<0.05, ^**p<0.01, ^***p<0.005.