Figure 5. Influence of mouse IL-7 on the survival of porcine T cells. Porcine PBMCs and mouse splenocytes were cultured and treated with either media alone or different concentrations of mouse IL-7 for 5 days. The frequency and number of PI⁺ or PI⁻ cells were measured by flow cytometry. (A) The fold change of percentage of propidium iodide positive (PI⁺) lymphocytes are plotted at the indicated concentration of mouse IL-7. The percentage of PI⁺ porcine CD4⁺ (B) or CD8⁺ (C) or CD4⁺CD8⁺ (D) T cells is shown. Mouse splenocytes were used as a positive control in this experiment. These experiments were done in quadruplicates, in 3 independent experiments. Error bars represent SD. The statistical significance was measured by t-test.

^*p<0.05, ^**p<0.01.