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. 2021 Jun 29;2021:5522291. doi: 10.1155/2021/5522291

Figure 2.

Figure 2

The effect of TSG-6 secreted by BMSCs on activated astrocytes. (a) qRT-PCR was utilized to detect TSG-6 expression in BMSC cells. (b) Western blot analysis of the expression of TSG-6 in BMSC cells. (c) qRT-PCR was utilized to test the expression levels of occludin, claudin 3, ZO-1, and iNOS in activated astrocytes. (d) Western blot was used to measure the protein expression levels of occludin, claudin 3, ZO-1, and iNOS in astrocytes. (e) TUNEL assay was used to detect astrocyte apoptosis (scale bar, 100 μm). (f) Invasion of astrocytes was examined by transwell assay (scale bar, 50 μm). BMSCs (medium containing BMSCs (TNF-α activation group)), si − NC + BMSCs (medium containing BMSCs (NC siRNA + TNF − α activation group)), and si − TSG − 6 + BMSCs (medium containing BMSCs (siRNA − TSG − 6 + TNF − α activation group)). ∗Compared with the control group, P < 0.05; #compared with the si − NC + BMSC group, P < 0.05. The above experimental results were all measurement data. Data were expressed as the mean ± SD. One-way ANOVA and Tukey's test were used only for the data comparison between multiple groups.