Figure 3.

TSG-6 secreted by BMSCs inhibited the activation of the NF-κB/MAPK signaling pathway. (a) qRT-PCR measured the expression of the NF-κB/MAPK signaling pathway. (b) Western blot observed the protein expression of the NF-κB/MAPK signaling pathway. BMSCs (medium containing BMSCs (TNF-α activation group)), si − NC + BMSCs (medium containing BMSCs (NC siRNA + TNF − α activation group)), and si − TSG − 6 + BMSCs (medium containing BMSCs (siRNA − TSG − 6 + TNF − α activation group)). ^∗Compared with the control group, P < 0.05; ^#compared with the si − NC + BMSC group, P < 0.05. The above experimental results were all measurement data. Data were expressed as the mean ± SD. One-way ANOVA and Tukey's test were used only for the data comparison between multiple groups.