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. 2021 Jun 29;2021:5522291. doi: 10.1155/2021/5522291

Figure 6.

Figure 6

BMSCs regulate astrocytes through TSG-6 to treat rats suffering from SAH. (a) The expression of TSG-6 and iNOS was measured by qRT-PCR and Western blot. (b) qRT-PCR and Western blot were used to measure the expression level of the NF-κB/MAPK signaling pathway. (c) ELISA was used to test the level of inflammatory factors secreted by activated astrocytes. (d) Evans blue detected changes in BBB permeability. (e) NO fluorescent probe analyzed the amount of NO production in the cell. (f) Determination of the ONOO− level in rat serum by ELISA. (g) Double immunofluorescence staining was used to measure the expression of iNOS (red) and GAFP (green). BMSCs (medium containing BMSCs (TNF-α activation group)), si − NC + BMSCs (medium containing BMSCs (NC siRNA + TNF − α activation group)), and si − TSG − 6 + BMSCs (medium containing BMSCs (siRNA − TSG − 6 + TNF − α activation group)). ∗Compared with the sham group, P < 0.05; #compared with the si − NC + BMSC group, P < 0.05. The above experimental results were all measurement data. Data are expressed as the mean ± SD. One-way ANOVA and Tukey's test were used for the data comparison between multiple groups.